SAM spec:
9. TLEN: signed observed Template LENgth. If all segments are mapped to the same reference, the
unsigned observed template length equals the number of bases from the leftmost mapped base
to the rightmost mapped base. The leftmost segment has a plus sign and the rightmost has a
minus sign. The sign of segments in the middle is undened. It is set as 0 for single-segment
template or when the information is unavailable.
: TLEN은 picard에서 insert size를 계산할 때 사용하는 값으로 insert size로도 대표된다.
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